Search for breast cancer stem cells that demonstrate treatment resistance

Recent advances in biology have revealed that cancer is not a monoclonal mass, but rather that the cancer cell masses also have a hierarchy similar to that of normal tissue. The cancer stem cells at the top of this have self-renewal and hierarchy regenerative abilities. We are introducing p53 genes that can induce doxycycline in human breast cancer cultured cells, creating a cell cluster that artificially causes apoptosis. At this time, cells that survive apoptosis by the manifestation of p53 show a resemblance to cancer stem cells. Through detailed analysis of these cells, we are aiming to develop new drug treatment methods.